Quantification of major royal jelly protein 1 in fresh royal jelly by indirect enzyme-linked immunosorbent assay.

Authors :: Yamaguchi K
He S
Li Z
Murata K
Hitomi N
Mozumi M
Ariga R
Enomoto T
Source :: Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2013; Vol. 77 (6), pp. 1310-2. Date of Electronic Publication: 2013 Jun 07.
Publication Year :: 2013
Abstract: Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12-4.67 g/100 g in different company's royal jelly, which almost agreed with that of a hexametric form of MRJP1 (apisin) measured by high performance liquid chromatography. These results suggest that MRJP1 exists mainly as apisin in royal jelly.

Subjects :: Animals
Annexin A2 chemistry
Chromatography, High Pressure Liquid
Enzyme-Linked Immunosorbent Assay
Fatty Acids metabolism
Glycoproteins chemistry
Glycoproteins immunology
Insect Proteins chemistry
Insect Proteins immunology
Antibodies immunology
Fatty Acids chemistry
Glycoproteins isolation & purification
Insect Proteins isolation & purification

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