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[Effects of soothing liver and invigorating spleen recipes on the mRNA and protein expression of TLR4 in hepatic tissues of rats with NASH].
- Source :
-
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2013 Jan; Vol. 36 (1), pp. 78-84. - Publication Year :
- 2013
-
Abstract
- Objective: To research the effects of soothing liver and invigorating spleen recipes on expression of TLR4 mRNA and protein expression in hepatic tissue of rats with non-alcoholic steatohepatitis and its mechanism.<br />Methods: 72 male SD rats were randomly divided into 8 groups: normal group, model group, high-dose soothing liver group (receiving gavage of Chaihu Shugan Powder 9. 6 g/kg), low-dose soothing liver group (receiving gavage of Chaihu Shugan Powder 3.2 g/kg), high-dose invigorating spleen group (receiving gavage of Shen Ling Baizhu Powder 30 g/kg), low-dose invigorating spleen group (receiving gavage of Shen Ling Baizhu Powder 10 g/kg), high-dose integrated Group (receiving gavage of Chaihu Shugan Powder and Shen Ling Baizhu Powder combination recipes 39.6 g/kg), low-dose integrated Group (receiving gavage of Chaihu Shugan Powder and Shen Ling Baizhu Powder combination recipes 13.2 g/kg), 9 rats were in each group. Used high fat diet (10 mL/kg) to establish experiment model of NASH rat. At the end of the sixteenth weeks, the levels of serum lipids, liver lipids and serum aminotransferase were measured by automatic biochemical analyzer; Liver pathology was analyzed by HE and Oil red O staining; TLR4 mRNA was assayed by real-time fluorescent quantitative polymerase chain reaction (RT Q-PCR); TLR4 protein was detected by Western blot.<br />Results: Compared with normal group, the levels of TC, LDL-C in the serum, TC,TG as well as the expression of TLR4 mRNA and protein in the hepatic tissue were dramatically increased in model group (P < 0.01). Compared with the model group, the levels of serum lipids, liver lipids, the expression of TLR4 mRNA and protein in the hepatic tissue were decreased in each treatment group (P < 0.05 or P < 0.01).<br />Conclusion: Soothing liver and invigorating spleen recipes can inhibit hepatic TLR4 expression, that may be one of their therapeutic mechanisms. There is much difference between high-does and low-does treatment groups in various testing items, which shows that there is does-effect relationship in intervention NASH.
- Subjects :
- Animals
Cholesterol blood
Disease Models, Animal
Dose-Response Relationship, Drug
Down-Regulation
Drug Combinations
Drugs, Chinese Herbal administration & dosage
Fatty Liver pathology
Hepatocytes metabolism
Hepatocytes pathology
Hypolipidemic Agents administration & dosage
Liver metabolism
Liver pathology
Liver Function Tests
Male
Non-alcoholic Fatty Liver Disease
Plants, Medicinal chemistry
RNA, Messenger genetics
RNA, Messenger metabolism
Random Allocation
Rats
Rats, Sprague-Dawley
Toll-Like Receptor 4 genetics
Triglycerides blood
Drugs, Chinese Herbal pharmacology
Fatty Liver metabolism
Hypolipidemic Agents pharmacology
Toll-Like Receptor 4 metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1001-4454
- Volume :
- 36
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
- Publication Type :
- Academic Journal
- Accession number :
- 23750414