[Optimization of extraction of Clerodendranthus spicatus using response surface methodology].

Objective: To obtain the optimal conditions of extraction of Clerodendranthus spicatus and provide the theoretical foundation for its further processing and utilization.
Methods: On the basis of the single factor test, response surface methodology was applied to optimize the extraction conditions.
Results: The results showed that extraction time, water-feed ratio, ethanol concentration and extraction temperature all had significant effects on the extraction rate of polysaccharides. The optimal extraction time was 3h, solid-liquid ratio was 50:1, ethanol concentration was 30% and extraction temperature was 80 degrees C. Under these optimized conditions, the extraction rate was 27.71%.
Conclusion: The extraction technology is simple, reliable and highly predictive.