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Disparate hypervariable region-1 of mitochondrial DNA did not induce skin allograft rejection in cloned porcine models.
- Source :
-
Transplantation proceedings [Transplant Proc] 2013 Jun; Vol. 45 (5), pp. 1787-91. - Publication Year :
- 2013
-
Abstract
- Introduction: Alloantigen recognition in skin transplantation is the bane for surgeons. Several studies have mainly focused on the immunogenicity of major histocompatibility (MHC) antigens and H-Y minor histocompatibility antigens. However, the roles of the mitochondrial DNA (mtDNA) encorded miHA have not been identified. Therefore, we sought to address the antigenicity of the hypervariable region 1 (HV-1) of mtDNA in skin transplantation using cloned pig models.<br />Methods: Swine leukocyte antigen and HV-1 of mtDNA were analyzed using sequencing methods. Skin transplantation was performed between MHC-matched, mtDNA-mismatched cloned miniature pigs. Full-thickness skin was grafted between cloned pigs without any immunosuppressants. The grafted tissues were observed for 3 months and evaluated histologically.<br />Results: The cloned pigs shared identical MHC but mtDNA mismatched at 9 positions. Skin grafts between the cloned pigs were accepted and hair growth maintained, whereas MHC-mismatched grafts showed acute rejection within 7 days after transplantation and were replaced by hairless scar tissue.<br />Conclusions: HV-1 disparate skin grafts were not recognized as alloantigenic by MHC-matched cloned pigs.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-2623
- Volume :
- 45
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Transplantation proceedings
- Publication Type :
- Academic Journal
- Accession number :
- 23769044
- Full Text :
- https://doi.org/10.1016/j.transproceed.2013.01.023