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Basophils help establish protective immunity induced by irradiated larval vaccination for filariasis.
- Source :
-
Vaccine [Vaccine] 2013 Aug 12; Vol. 31 (36), pp. 3675-82. Date of Electronic Publication: 2013 Jun 15. - Publication Year :
- 2013
-
Abstract
- Basophils are increasingly recognized as playing important roles in the immune response toward helminths. In this study, we evaluated the role of basophils in vaccine-mediated protection against filariae, tissue-invasive parasitic nematodes responsible for diseases such as elephantiasis and river blindness. Protective immunity and immunological responses were assessed in BALB/c mice vaccinated with irradiated L3 stage larvae and depleted of basophils with weekly injections of anti-CD200R3 antibody. Depletion of basophils after administration of the vaccination regimen but before challenge infection did not alter protective immunity. In contrast, basophil depletion initiated prior to vaccination and continued after challenge infection significantly attenuated the protective effect conferred by vaccination. Vaccine-induced cellular immune responses to parasite antigen were substantially decreased in basophil-depleted mice, with significant decreases in CD4(+) T-cell production of IL-4, IL-5, IL-10, and IFN-γ. Interestingly, skin mast cell numbers, which increased significantly after vaccination with irradiated L3 larvae, were unchanged after vaccination in basophil-depleted mice. These findings demonstrate that basophils help establish the immune responses responsible for irradiated L3 vaccine protection.<br /> (Published by Elsevier Ltd.)
- Subjects :
- Animals
Antibodies, Helminth blood
Antibodies, Monoclonal administration & dosage
CD4-Positive T-Lymphocytes immunology
Female
Filariasis immunology
Filarioidea radiation effects
Immunity, Cellular
Immunoglobulin E blood
Interferon-gamma immunology
Interleukins immunology
Larva immunology
Larva radiation effects
Lymphocyte Depletion
Mast Cells immunology
Mice
Mice, Inbred BALB C
Basophils immunology
Filariasis prevention & control
Filarioidea immunology
Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 31
- Issue :
- 36
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 23777951
- Full Text :
- https://doi.org/10.1016/j.vaccine.2013.06.010