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Nonenzymatic labeling of 5-hydroxymethylcytosine in nanopore sequencing.
- Source :
-
Chembiochem : a European journal of chemical biology [Chembiochem] 2013 Jul 22; Vol. 14 (11), pp. 1289-90. Date of Electronic Publication: 2013 Jun 18. - Publication Year :
- 2013
-
Abstract
- Labeling chemically for a "single" result: A one-step, nonenzymatic 5hmC thiolation method mediated by bisulfite is highlighted. The 5hmC base can be chemically labeled in single-stranded DNA and detected by using nanopore sequencing.<br /> (Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Details
- Language :
- English
- ISSN :
- 1439-7633
- Volume :
- 14
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Chembiochem : a European journal of chemical biology
- Publication Type :
- Academic Journal
- Accession number :
- 23780928
- Full Text :
- https://doi.org/10.1002/cbic.201300342