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Lysophosphatidic acid increases the production of pivotal mediators of decidualization and vascularization in the rat uterus.
- Source :
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Placenta [Placenta] 2013 Sep; Vol. 34 (9), pp. 751-6. Date of Electronic Publication: 2013 Jun 20. - Publication Year :
- 2013
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Abstract
- Introduction: The decidual reaction and the formation of new vessels in the uterus are two crucial processes during embryo implantation. Previously, we observed that lysophosphatidic acid (LPA) increases cyclooxygenase-2 derived - prostaglandin E2 production during implantation in the rat uterus and that it augments the expression of decidualization (IGFBP-1) and vascularization (IL-10) markers. Both cyclooxygenase and nitric oxide synthase (NOS) are known enzymes involved in these processes. Thus, we became interested in studying which factors contribute to LPA receptor-specific role during the decidual and the vascular reaction at implantation.<br />Methods: We adopted a pharmacological approach in vitro incubating the uterus from rats on day 5 of gestation (day of implantation) with LPA, DGPP (a highly selective antagonist of LPA3, an LPA receptor) and cyclooxygenase and NOS selective and non-selective inhibitors. We determined NOS activity, prostaglandin E2 production and IGFBP-1 and IL-10 expression to evaluate decidualization and vascularization.<br />Results: We observed that LPA augmented the activity of the inducible NOS isoform through LPA1/LPA3. Inducible NOS activity participated in the induction of cyclooxygenase-2/prostaglandin E2 increase stimulated by LPA. Also, cyclooxygenase-2 derived prostaglandins mediated LPA-stimulatory action on NOS activity. Both cyclooxygenase-2 and inducible NOS mediated LPA effect on IGFBP-1 and IL-10 expression.<br />Conclusions: These results suggest the participation of LPA/LPA3 in the production of crucial molecules involved in vascularization and decidualization, two main processes that prepare the uterine milieu for embryo invasion during implantation.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Biomarkers metabolism
Cyclooxygenase 2 chemistry
Cyclooxygenase 2 metabolism
Cyclooxygenase 2 Inhibitors pharmacology
Decidua cytology
Decidua drug effects
Decidua metabolism
Dinoprostone metabolism
Enzyme Inhibitors pharmacology
Female
Gene Expression Regulation, Developmental drug effects
Insulin-Like Growth Factor Binding Protein 1 genetics
Insulin-Like Growth Factor Binding Protein 1 metabolism
Interleukin-10 genetics
Interleukin-10 metabolism
Lysophospholipids antagonists & inhibitors
Nitric Oxide Synthase Type II antagonists & inhibitors
Nitric Oxide Synthase Type II metabolism
Pregnancy
Protein Isoforms antagonists & inhibitors
Protein Isoforms metabolism
Rats
Rats, Wistar
Receptors, Lysophosphatidic Acid antagonists & inhibitors
Uterus cytology
Uterus drug effects
Uterus metabolism
Decidua blood supply
Embryo Implantation drug effects
Lysophospholipids metabolism
Placentation
Receptors, Lysophosphatidic Acid metabolism
Signal Transduction drug effects
Uterus blood supply
Subjects
Details
- Language :
- English
- ISSN :
- 1532-3102
- Volume :
- 34
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Placenta
- Publication Type :
- Academic Journal
- Accession number :
- 23790320
- Full Text :
- https://doi.org/10.1016/j.placenta.2013.06.001