Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development.

Authors :: Ong EB
Anthony AA
Ismail A
Ismail A
Lim TS
Source :: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2013 Sep; Vol. 77 (1), pp. 87-9. Date of Electronic Publication: 2013 Jun 20.
Publication Year :: 2013
Abstract: The hemolysin (HlyE) protein of Salmonella enterica serovar Typhi was reported to be antigenic. This work describes the cloning, expression, and purification of a hexahistidine-tagged HlyE protein under native conditions. Immunoblot analysis and a competitive enzyme-linked immunosorbent assay using sera from typhoid patients showed the presence of HlyE-specific antibodies in circulation.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)

Subjects :: Cloning, Molecular
Enzyme-Linked Immunosorbent Assay methods
Gene Expression
Humans
Immunoblotting methods
Recombinant Proteins genetics
Recombinant Proteins isolation & purification
Salmonella typhi genetics
Antibodies, Bacterial blood
Antigens, Bacterial genetics
Antigens, Bacterial isolation & purification
Clinical Laboratory Techniques methods
Hemolysin Proteins genetics
Hemolysin Proteins isolation & purification
Salmonella typhi immunology
Typhoid Fever diagnosis

Full Text Access

Tools