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[Expression of Id1 and Id3 in endometrial carcinoma and their roles in regulating biological behaviors of endometrial carcinoma cells in vitro].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2013 Jun; Vol. 33 (6), pp. 812-8. - Publication Year :
- 2013
-
Abstract
- Objective: To investigate the expression of inhibitor of DNA differentiation/DNA binding 1 (Id1) and Id3 in endometrial carcinoma and explore their roles in regulating the proliferation, invasion, migration and adhesion of endometrial carcinoma cells in vitro.<br />Methods: Id1 and Id3 expression in 4 fresh endometrial cancer tissue specimens and matched adjacent tissues were detected using Western blotting. Two endometrial cancer cell lines, HEC-1-B and RL-952, were both divided into 4 groups, namely the untreated group, blank virus group, promoter group and Id1/Id3 double-knockdown group, and their expressions of MMP2, CXCR4 and P21 were detected by qRT-PCR and Western blotting. The proliferation, invasion, migration and adhesion of the cells were evaluated with MTT, Transwell, wound-healing, and adhesion assays.<br />Results: Endometrial carcinoma tissues showed significantly higher Id1 and Id3 expression than the adjacent tissues (P<0.05). In the two endometrial carcinoma cell lines, Id1/Id3 double-knockdown significantly decreased MMP2 and CXCR4 expression and increased P21 expression at both mRNA and protein levels (P<0.05), and resulted in suppressed cell proliferation, invasion, migration and adhesion.<br />Conclusion: Id1 and Id3 expressions are up-regulated in endometrial carcinoma to promote the proliferation, invasion, migration and adhesion of the tumor cells by increasing MMP2 and CXCR4 expression and reducing P21 expression. Therapies targeting Id1/Id3 can be a novel strategy for treatment of endometrial carcinoma.
- Subjects :
- Cell Adhesion
Cell Line, Tumor
Cell Movement
Cell Proliferation
Endometrial Neoplasms pathology
Female
Humans
Matrix Metalloproteinase 2 metabolism
Proto-Oncogene Proteins p21(ras) metabolism
RNA Interference
Receptors, CXCR4 metabolism
Endometrial Neoplasms metabolism
Inhibitor of Differentiation Protein 1 metabolism
Inhibitor of Differentiation Proteins metabolism
Neoplasm Proteins metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 33
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 23803189