Evaluation of cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate, and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme for recovery of Clostridium difficile isolates from fecal samples.

Authors :: Tyrrell KL
Citron DM
Leoncio ES
Merriam CV
Goldstein EJ
Source :: Journal of clinical microbiology [J Clin Microbiol] 2013 Sep; Vol. 51 (9), pp. 3094-6. Date of Electronic Publication: 2013 Jun 26.
Publication Year :: 2013
Abstract: Cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate (CCFA-HT), and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme (CCMB-TAL) were compared for recovery of Clostridium difficile from 120 stool specimens. Compared to CCFA, CCFA-HT enhanced C. difficile growth and improved recovery by 4%. In a separate study, 9% (8/91) of stool samples previously C. difficile negative on plate medium were C. difficile positive when cultured in CCMB-TAL.

Subjects :: Agar
Animals
Anti-Infective Agents metabolism
Cefoxitin metabolism
Cycloserine metabolism
Erythrocytes metabolism
Fructose metabolism
Horses
Humans
Mannitol metabolism
Muramidase metabolism
Taurocholic Acid metabolism
Clostridioides difficile isolation & purification
Clostridium Infections diagnosis
Clostridium Infections microbiology
Culture Media chemistry
Feces microbiology

Full Text Access

Tools