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Lipid in skeletal muscle myotubes is associated to the donors' insulin sensitivity and physical activity phenotypes.

Authors :
Bajpeyi S
Myrland CK
Covington JD
Obanda D
Cefalu WT
Smith SR
Rustan AC
Ravussin E
Source :
Obesity (Silver Spring, Md.) [Obesity (Silver Spring)] 2014 Feb; Vol. 22 (2), pp. 426-34. Date of Electronic Publication: 2013 Sep 10.
Publication Year :
2014

Abstract

Objective: This study investigated the relationship between in vitro lipid content in myotubes and in vivo whole body phenotypes of the donors such as insulin sensitivity, intramyocellular lipids (IMCL), physical activity, and oxidative capacity.<br />Design and Methods: Six physically active donors were compared to six sedentary lean and six T2DM. Lipid content was measured in tissues and myotubes by immunohistochemistry. Ceramides, triacylglycerols, and diacylglycerols (DAGs) were measured by LC-MS-MS and GC-FID. Insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp (80 mU min⁻¹ m⁻²), maximal mitochondrial capacity (ATPmax) by ³¹P-MRS, physical fitness by VO₂max and physical activity level (PAL) by accelerometers.<br />Results: Myotubes cultured from physically active donors had higher lipid content (0.047 ± 0.003 vs. 0.032 ± 0.001 and 0.033 ± 0.001AU; P < 0.001) than myotubes from lean and T2DM donors. Lipid content in myotubes was not associated with IMCL in muscle tissue but importantly, correlated with in vivo measures of ATPmax (r = 0.74; P < 0.001), insulin sensitivity (r = 0.54; P < 0.05), type-I fibers (r = 0.50; P < 0.05), and PAL (r = 0.92; P < 0.0001). DAGs and ceramides in myotubes were inversely associated with insulin sensitivity (r = -0.55, r = -0.73; P < 0.05) and ATPmax (r = -0.74, r = -0.85; P < 0.01).<br />Conclusions: These results indicate that cultured human myotubes can be used in mechanistic studies to study the in vitro impact of interventions on phenotypes such as mitochondrial capacity, insulin sensitivity, and physical activity.<br /> (Copyright © 2013 The Obesity Society.)

Details

Language :
English
ISSN :
1930-739X
Volume :
22
Issue :
2
Database :
MEDLINE
Journal :
Obesity (Silver Spring, Md.)
Publication Type :
Academic Journal
Accession number :
23818429
Full Text :
https://doi.org/10.1002/oby.20556