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Investigation of macrophage polarization using bone marrow derived macrophages.
- Source :
-
Journal of visualized experiments : JoVE [J Vis Exp] 2013 Jun 23 (76). Date of Electronic Publication: 2013 Jun 23. - Publication Year :
- 2013
-
Abstract
- The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
- Subjects :
- Animals
Bone Marrow Cells cytology
Bone Marrow Cells drug effects
Cell Differentiation drug effects
Cell Polarity drug effects
Cell Polarity physiology
Granulocyte-Macrophage Colony-Stimulating Factor pharmacology
Hematopoietic Stem Cells cytology
Hematopoietic Stem Cells drug effects
Macrophage Activation drug effects
Macrophage Activation physiology
Macrophage Colony-Stimulating Factor pharmacology
Macrophages drug effects
Mice
Cytological Techniques methods
Macrophages cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1940-087X
- Issue :
- 76
- Database :
- MEDLINE
- Journal :
- Journal of visualized experiments : JoVE
- Publication Type :
- Academic Journal
- Accession number :
- 23851980
- Full Text :
- https://doi.org/10.3791/50323