Back to Search
Start Over
Release of soluble CD30 after allogeneic stimulation is mediated by memory T cells and regulated by IFN-γ and IL-2.
- Source :
-
Transplantation [Transplantation] 2013 Jul 27; Vol. 96 (2), pp. 154-61. - Publication Year :
- 2013
-
Abstract
- Background: Membrane CD30 is an important costimulatory molecule for activated T lymphocytes, and serum level of soluble CD30 (sCD30) is considered a marker for predicting outcome in kidney transplantation.<br />Methods: We investigated the kinetics of CD30 expression on CD4 and CD8 T-cell populations and the source of sCD30 during alloimmune responses in vitro. The effect of neutralizing antibodies against interferon (IFN)-γ and other cytokines on sCD30 release and the involvement of metalloproteinases ADAM10 and ADAM17/TACE that are responsible for sCD30 shedding were also assessed. Memory phenotypes and CD30 expression on allostimulated CD3 lymphocytes were evaluated in dialysis patients and matched controls.<br />Results: Allogeneic stimulation resulted in conversion of naive responder cells to central memory CD4 cells (P<0.001 at 96 hr) and effector CD8 cells (P<0.01 at 120 hr), which was accompanied by increased CD30 expression. Release of sCD30 was attributed mainly to central memory cells, and neutralization of IFN-γ (P<0.001) and interleukin (IL)-2 (P<0.001) impaired the release of sCD30 during allostimulation but did not alter the levels of ADAM10 and ADAM17/TACE. CD30 expression was modulated in dialysis patients in a similar way as in healthy controls.<br />Conclusions: Allostimulation results in the up-regulation of the T-cell activation marker CD30 on CD4 as well as CD8 memory T cells and increased release of sCD30 from these cells in an IFN-γ- and IL-2-dependent manner. These results may explain clinical findings on the suitability of sCD30 and IFN-γ- and IL-2-producing T cells for immune monitoring of kidney transplant recipients before and after transplantation.
- Subjects :
- ADAM Proteins immunology
ADAM10 Protein
ADAM17 Protein
Adult
Amyloid Precursor Protein Secretases immunology
Female
Flow Cytometry
Humans
Immunophenotyping
Isoantigens immunology
Ki-1 Antigen blood
Kidney Transplantation immunology
Lymphocyte Activation immunology
Male
Membrane Proteins immunology
Middle Aged
Solubility
Up-Regulation immunology
CD4-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes immunology
Immunologic Memory immunology
Interferon-gamma immunology
Interleukin-2 immunology
Ki-1 Antigen immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1534-6080
- Volume :
- 96
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 23857000
- Full Text :
- https://doi.org/10.1097/TP.0b013e318296fd69