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Mannitol utilisation is required for protection of Staphylococcus aureus from human skin antimicrobial fatty acids.
- Source :
-
PloS one [PLoS One] 2013 Jul 04; Vol. 8 (7), pp. e67698. Date of Electronic Publication: 2013 Jul 04 (Print Publication: 2013). - Publication Year :
- 2013
-
Abstract
- Mannitol (Mtl) fermentation, with the subsequent production of acid, is a species signature of Staphylococcus aureus, and discriminates it from most other members of the genus. Inactivation of the gene mtlD, encoding Mtl-1-P dehydrogenase was found to markedly reduce survival in the presence of the antimicrobial fatty acid, linoleic acid. We demonstrate that the sugar alcohol has a potentiating action for this membrane-acting antimicrobial. Analysis of cellular metabolites revealed that, during exponential growth, the mtlD mutant accumulated high levels of Mtl and Mtl-P. The latter metabolite was not detected in its isogenic parent strain or a deletion mutant of the entire mtlABFD operon. In addition, the mtlD mutant strain exhibited a decreased MIC for H2O2, however virulence was unaffected in a model of septic arthritis.
- Subjects :
- Animals
Bacterial Proteins metabolism
Humans
Hydrogen Peroxide pharmacology
Linoleic Acid pharmacology
Mice
Microbial Sensitivity Tests
Microbial Viability drug effects
Mutation
Operon
Staphylococcal Skin Infections microbiology
Staphylococcus aureus genetics
Staphylococcus aureus growth & development
Sugar Alcohol Dehydrogenases deficiency
Virulence
Bacterial Proteins genetics
Gene Expression Regulation, Bacterial
Mannitol metabolism
Skin microbiology
Staphylococcus aureus metabolism
Sugar Alcohol Dehydrogenases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23861785
- Full Text :
- https://doi.org/10.1371/journal.pone.0067698