Back to Search
Start Over
Expression of three novel cytochrome P450 (CYP) and antioxidative genes from the polychaete, Perinereis nuntia exposed to water accommodated fraction (WAF) of Iranian crude oil and benzo[a]pyrene.
- Source :
-
Marine environmental research [Mar Environ Res] 2013 Sep; Vol. 90, pp. 75-84. Date of Electronic Publication: 2013 Jun 21. - Publication Year :
- 2013
-
Abstract
- To report a novel CYP genes and to evaluate its potency as a biomarker for oil pollution, we cloned three CYP genes and measured their expression profiles under controlled lab conditions using real-time reverse transcription PCR (real-time RT-PCR) after exposure of the water accommodated fraction (WAF) of Iranian crude oil and benzo[α]pyrene (B[α]P) as a positive control. Of these, CYP432A1 (CYP3 clan) gene was significantly induced by B[α]P exposure, indicating that the CYP3 clan gene would play an important role in polycyclic aromatic hydrocarbon (PAH) metabolisms, particularly for B[α]P in this species. However, the Perinereis nuntia CYP431A1 mRNA, a CYP2 clan gene, was sensitively expressed to WAF exposure with other two CYP genes. As one of Phase II detoxification enzymes, the glutathione S-transferase (GST) genes also upregulated with other antioxidant genes (SOD and CAT), indicating that WAF-exposed P. nuntia was properly responding to this kind of chemical stress. Thus, three CYP genes from the polychaete, P. nuntia have a potential as a biomarker in monitoring of the marine sediment after an oil spill accident.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Biomarkers metabolism
Cytochrome P-450 Enzyme System chemistry
Cytochrome P-450 Enzyme System metabolism
Environmental Monitoring
Molecular Sequence Data
Phylogeny
Polychaeta drug effects
Polychaeta metabolism
Polymerase Chain Reaction
Sequence Alignment
Benzo(a)pyrene toxicity
Cytochrome P-450 Enzyme System genetics
Environmental Exposure
Gene Expression Regulation, Enzymologic
Petroleum toxicity
Polychaeta genetics
Water Pollutants, Chemical toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0291
- Volume :
- 90
- Database :
- MEDLINE
- Journal :
- Marine environmental research
- Publication Type :
- Academic Journal
- Accession number :
- 23871518
- Full Text :
- https://doi.org/10.1016/j.marenvres.2013.05.014