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The improved PCR of the fstA (ferric siderophore receptor) gene differentiates the fish pathogen Aeromonas salmonicida from other Aeromonas species.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2013 Oct 25; Vol. 166 (3-4), pp. 659-63. Date of Electronic Publication: 2013 Jul 09. - Publication Year :
- 2013
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Abstract
- The members of the genus Aeromonas are autochthonous of aquatic ecosystems and several species have been associated to septicaemia, ulcerative and haemorrhagic diseases in fish, causing significant mortality in both wild and farmed, freshwater and marine fish species. The species Aeromonas salmonicida is generally recognized as the most important fish pathogen responsible for epidemic outbreaks of furunculosis in salmonids, also being able to produce infections in other cultured fish such as turbot, halibut, sea bream or goldfish. New species, i.e. Aeromonas aquariorum, Aeromonas tecta and Aeromonas piscicola, have recently been discovered and isolated from diseased fish. The species A. piscicola and Aeromonas bestiarum are practically impossible to differentiate phenotypically and genetically (when using the 16S rRNA gene) from each other and from A. salmonicida. In the present study, two previously described PCR protocols, based on the fstA and gyrB genes, for the specific detection of A. salmonicida were re-evaluated with the type strains of all Aeromonas species and with a set of A. piscicola and A. bestiarum strains. Contrary to what had been published previously it was demonstrated that the gyrB-PCR is not specific for A. salmonicida because of cross-reactions with other Aeromonas species. However, in agreement with previous results, A. salmonicida was detected on the basis of the fstA-PCR, for which an improved protocol was proposed.<br /> (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Subjects :
- Aeromonas classification
Aeromonas genetics
Aeromonas salmonicida classification
Aeromonas salmonicida genetics
Animals
Fishes
Furunculosis microbiology
Polymerase Chain Reaction methods
RNA, Ribosomal, 16S genetics
Siderophores metabolism
Aeromonas isolation & purification
Aeromonas salmonicida isolation & purification
Bacterial Proteins genetics
Fish Diseases microbiology
Furunculosis veterinary
Polymerase Chain Reaction veterinary
Salmonidae microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2542
- Volume :
- 166
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 23890674
- Full Text :
- https://doi.org/10.1016/j.vetmic.2013.06.028