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[Salidroside protects cultured rat subventricular zone neural stem cells against hypoxia injury by inhibiting Bax, Bcl-2 and caspase-3 expressions].

Authors :
Qi C
Zhang J
Chen X
Zhang J
Yang P
Jiao Q
Zhang P
Lu HX
Liu Y
Source :
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2013 Jul; Vol. 33 (7), pp. 962-6.
Publication Year :
2013

Abstract

Objective: To explore the effects of salidroside (sal) on the expressions of Bcl-2, Bax and caspases-3 proteins in cultured rat subventricular zone (SVZ) neural stem cells (NSCs) exposed to hypoxia injury.<br />Methods: Primarily cultured SVZ NSCs from adult SD rats were incubated with salidroside (120 and 240 µmol/L) for 24 h prior to exposure to hypoxia. The cell viability was assessed with MTT assay, and the cell apoptosis was analyzed using TUNEL staining and flow cytometry. Western blotting was performed to detect the expressions of Bcl-2, Bax and caspase-3 in the cells.<br />Results: Salidroside pretreatment of the cells for 24 h resulted in an obvious resistance to hypoxia-induced cell apoptosis and decrement of cell viability (P<0.05). Salidroside also antagonized the effect of hypoxia exposure in lowering Bcl-2/Bax ratio apoptosis of rat neural stem cells and decreased the expression of caspases-3 protein (P<0.05).<br />Conclusion: Salidroside can significantly resist hypoxia-induced. The neuroprotective effect of salidroside may be related to the modulation of expressions of apoptosis-related proteins.

Details

Language :
Chinese
ISSN :
1673-4254
Volume :
33
Issue :
7
Database :
MEDLINE
Journal :
Nan fang yi ke da xue xue bao = Journal of Southern Medical University
Publication Type :
Academic Journal
Accession number :
23895833