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Generation of patient-specific induced neuronal cells using a direct reprogramming strategy.
- Source :
-
Stem cells and development [Stem Cells Dev] 2014 Jan 01; Vol. 23 (1), pp. 16-23. Date of Electronic Publication: 2013 Sep 17. - Publication Year :
- 2014
-
Abstract
- Direct reprogramming of human fibroblasts into functional neurons in vitro by defined factors provides an invaluable resource for regenerative medicine. However, clinical applications must consider the risk of immune rejection, thus patient-specific induced neuronal cells (iNCs) may serve as an ideal source for autologous cell replacement. In this study, we report a robust process for functional neuronal cells from the patients' scalp by lentiviral gene delivery of Ascl1, Myt1l, and Sox2. These three-factor iNCs are similar to human neuronal cells in morphology, surface antigens, gene expression, and electrophysiological characteristics. Our findings might provide a source of patient-specific functional neurons for cell therapy.
- Subjects :
- Animals
Basic Helix-Loop-Helix Transcription Factors genetics
Brain Injuries therapy
Cell Differentiation
Cell Transformation, Neoplastic
Cells, Cultured
Gene Expression
Gene Expression Regulation, Developmental
Homeodomain Proteins genetics
Humans
Ion Channel Gating
Ion Channels analysis
Mice
Mice, SCID
Nerve Regeneration
Nerve Tissue Proteins genetics
POU Domain Factors genetics
SOXB1 Transcription Factors genetics
Skin cytology
Transcription Factors genetics
Cell- and Tissue-Based Therapy
Cellular Reprogramming genetics
Fibroblasts physiology
Neural Stem Cells physiology
Neurons physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1557-8534
- Volume :
- 23
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Stem cells and development
- Publication Type :
- Academic Journal
- Accession number :
- 23947933
- Full Text :
- https://doi.org/10.1089/scd.2013.0131