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MiR-210 is induced by Oct-2, regulates B cells, and inhibits autoantibody production.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2013 Sep 15; Vol. 191 (6), pp. 3037-3048. Date of Electronic Publication: 2013 Aug 19. - Publication Year :
- 2013
-
Abstract
- MicroRNAs (MiRs) are small, noncoding RNAs that regulate gene expression posttranscriptionally. In this study, we show that MiR-210 is induced by Oct-2, a key transcriptional mediator of B cell activation. Germline deletion of MiR-210 results in the development of autoantibodies from 5 mo of age. Overexpression of MiR-210 in vivo resulted in cell autonomous expansion of the B1 lineage and impaired fitness of B2 cells. Mice overexpressing MiR-210 exhibited impaired class-switched Ab responses, a finding confirmed in wild-type B cells transfected with a MiR-210 mimic. In vitro studies demonstrated defects in cellular proliferation and cell cycle entry, which were consistent with the transcriptomic analysis demonstrating downregulation of genes involved in cellular proliferation and B cell activation. These findings indicate that Oct-2 induction of MiR-210 provides a novel inhibitory mechanism for the control of B cells and autoantibody production.
- Subjects :
- Animals
Autoantibodies immunology
B-Lymphocytes immunology
Cell Separation
Chromatin Immunoprecipitation
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Mice
Mice, Inbred C57BL
Mice, Transgenic
MicroRNAs immunology
Octamer Transcription Factor-2 immunology
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Transcriptome
Autoantibodies biosynthesis
B-Lymphocytes metabolism
Lymphocyte Activation immunology
MicroRNAs biosynthesis
Octamer Transcription Factor-2 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 191
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 23960236
- Full Text :
- https://doi.org/10.4049/jimmunol.1301289