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A universal DNA-based protein detection system.

Authors :
Tran TN
Cui J
Hartman MR
Peng S
Funabashi H
Duan F
Yang D
March JC
Lis JT
Cui H
Luo D
Source :
Journal of the American Chemical Society [J Am Chem Soc] 2013 Sep 25; Vol. 135 (38), pp. 14008-11. Date of Electronic Publication: 2013 Sep 11.
Publication Year :
2013

Abstract

Protein immune detection requires secondary antibodies which must be carefully selected in order to avoid interspecies cross-reactivity, and is therefore restricted by the limited availability of primary/secondary antibody pairs. Here we present a versatile DNA-based protein detection system using a universal adapter to interface between IgG antibodies and DNA-modified reporter molecules. As a demonstration of this capability, we successfully used DNA nano-barcodes, quantum dots, and horseradish peroxidase enzyme to detect multiple proteins using our DNA-based labeling system. Our system not only eliminates secondary antibodies but also serves as a novel method platform for protein detection with modularity, high capacity, and multiplexed capability.

Details

Language :
English
ISSN :
1520-5126
Volume :
135
Issue :
38
Database :
MEDLINE
Journal :
Journal of the American Chemical Society
Publication Type :
Academic Journal
Accession number :
23978265
Full Text :
https://doi.org/10.1021/ja405872g