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Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins.
- Source :
-
Journal of innate immunity [J Innate Immun] 2014; Vol. 6 (2), pp. 192-204. Date of Electronic Publication: 2013 Aug 27. - Publication Year :
- 2014
-
Abstract
- Heterorhabditis bacteriophora is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of the species Photorhabdus luminescens. We performed a genome-wide transcriptional analysis of the Drosophila response to EPN infection at the time point at which the nematodes reached the hemolymph either via the cuticle or the gut and the bacteria had started to multiply. Many of the most strongly induced genes have been implicated in immune responses in other infection models. Mapping of the complete set of differentially regulated genes showed the hallmarks of a wound response, but also identified a large fraction of EPN-specific transcripts. Several genes identified by transcriptome profiling or their homologues play protective roles during nematode infections. Genes that positively contribute to controlling nematobacterial infections encode: a homolog of thioester-containing complement protein 3, a basement membrane component (glutactin), a recognition protein (GNBP-like 3) and possibly several small peptides. Of note is that several of these genes have not previously been implicated in immune responses.<br /> (Copyright © 2013 S. Karger AG, Basel)
- Subjects :
- Animals
Animals, Genetically Modified
Cluster Analysis
Complement System Proteins genetics
Complement System Proteins metabolism
Drosophila Proteins genetics
Drosophila Proteins immunology
Drosophila Proteins metabolism
Drosophila melanogaster genetics
Drosophila melanogaster parasitology
Extracellular Matrix Proteins genetics
Extracellular Matrix Proteins metabolism
Gene Ontology
Hemocytes immunology
Hemocytes metabolism
Host-Parasite Interactions immunology
Host-Pathogen Interactions immunology
Larva genetics
Larva immunology
Larva parasitology
Luminescent Proteins genetics
Luminescent Proteins metabolism
Membrane Glycoproteins genetics
Membrane Glycoproteins immunology
Membrane Glycoproteins metabolism
Microscopy, Confocal
Oligonucleotide Array Sequence Analysis
Photorhabdus immunology
Photorhabdus physiology
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
Rhabditoidea microbiology
Rhabditoidea physiology
Complement System Proteins immunology
Drosophila melanogaster immunology
Extracellular Matrix Proteins immunology
Genome, Insect
Rhabditoidea immunology
Transcriptome immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1662-8128
- Volume :
- 6
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of innate immunity
- Publication Type :
- Academic Journal
- Accession number :
- 23988573
- Full Text :
- https://doi.org/10.1159/000353734