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Methods to quantify microRNAs in the Myc gene network for posttranscriptional gene repression.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 1012, pp. 135-44. - Publication Year :
- 2013
-
Abstract
- As a global transcription factor, Myc regulates both protein-coding genes and noncoding microRNA genes. Myc-activated or repressed miRNAs are involved in various pathways to affect tumorigenesis, mediate apoptosis, proliferation, angiogenesis, metastasis, and metabolism downstream of Myc. Functional characterization of miRNAs in the Myc network requires the accurate detection and quantification of miRNA expression levels. Here, we describe two widely used methodologies to determine miRNA expression, including miRNA real-time PCR and miRNA northern analysis.
- Subjects :
- MicroRNAs metabolism
Proto-Oncogene Proteins c-myc metabolism
Blotting, Northern methods
Gene Expression Regulation, Neoplastic
Gene Regulatory Networks
MicroRNAs genetics
Proto-Oncogene Proteins c-myc genetics
RNA Processing, Post-Transcriptional
Real-Time Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1012
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 24006063
- Full Text :
- https://doi.org/10.1007/978-1-62703-429-6_10