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Both PAX4 and MAFA are expressed in a substantial proportion of normal human pancreatic alpha cells and deregulated in patients with type 2 diabetes.
- Source :
-
PloS one [PLoS One] 2013 Aug 27; Vol. 8 (8), pp. e72194. Date of Electronic Publication: 2013 Aug 27 (Print Publication: 2013). - Publication Year :
- 2013
-
Abstract
- Pax4 and MafA (v-maf musculoaponeurotic fibrosarcoma oncogene homolog A) are two transcription factors crucial for normal functions of islet beta cells in the mouse. Intriguingly, recent studies indicate the existence of notable difference between human and rodent islet in terms of gene expression and functions. To better understand the biological role of human PAX4 and MAFA, we investigated their expression in normal and diseased human islets, using validated antibodies. PAX4 was detected in 43.0±5.0% and 39.1±4.0% of normal human alpha and beta cells respectively. We found that MAFA, detected in 88.3±6.3% insulin(+)cells as in the mouse, turned out to be also expressed in 61.2±6.4% of human glucagons(+) cells with less intensity than in insulin(+) cells, whereas MAFB expression was found not only in the majority of glucagon(+) cells (67.2±7.6%), but also in 53.6±10.5% of human insulin(+) cells. Interestingly, MAFA nuclear expression in both alpha and beta cells, and the percentage of alpha cells expressing PAX4 were found altered in a substantial proportion of patients with type 2 diabetes. Both MAFA and PAX4 display, therefore, a distinct expression pattern in human islet cells, suggesting more potential plasticity of human islets as compared with rodent islets.
- Subjects :
- Adolescent
Adult
Aged
Animals
Case-Control Studies
Cell Nucleus metabolism
Cells, Cultured
Diabetes Mellitus, Type 2 pathology
Female
Gene Expression
Gene Expression Regulation
Homeodomain Proteins genetics
Humans
Insulin-Secreting Cells metabolism
Maf Transcription Factors, Large genetics
Male
Mice
Middle Aged
Obesity metabolism
Paired Box Transcription Factors genetics
Pancreas metabolism
Pancreas pathology
Diabetes Mellitus, Type 2 metabolism
Glucagon-Secreting Cells metabolism
Homeodomain Proteins metabolism
Maf Transcription Factors, Large metabolism
Paired Box Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24013263
- Full Text :
- https://doi.org/10.1371/journal.pone.0072194