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High-throughput label-free detection of DNA hybridization and mismatch discrimination using interferometric reflectance imaging sensor.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 1039, pp. 181-200. - Publication Year :
- 2013
-
Abstract
- Optical label-free biosensors have demonstrated advantages over fluorescent-based detection methods by allowing accurate quantification while also being capable of measuring dynamic bimolecular interactions. A simple, high-throughput, solid-phase, and label-free technique, interferometric reflectance imaging sensor (IRIS), can quantify the mass density of DNA with pg/mm(2) sensitivity by measuring the optical path difference. We present the design of the IRIS instrument and complementary microarrays that can be used to perform a quantitative analysis of DNA microarrays. Finally, we present methods to accurately calculate the hybridization efficiency and identify SNPs from dynamic measurements, as well as supporting software algorithms needed for robust data processing.
- Subjects :
- Base Pair Mismatch
Base Sequence
DNA Probes chemistry
DNA Probes genetics
DNA, Single-Stranded chemistry
DNA, Single-Stranded genetics
Genotyping Techniques
Interferometry
Light
Oligonucleotide Array Sequence Analysis
Polymorphism, Single Nucleotide
Biosensing Techniques
High-Throughput Screening Assays
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1039
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 24026697
- Full Text :
- https://doi.org/10.1007/978-1-62703-535-4_16