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High-throughput functional microRNA profiling using recombinant AAV-based microRNA sensor arrays.

Authors :
Tian W
Dong X
Wu X
Wu Z
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 1039, pp. 261-77.
Publication Year :
2013

Abstract

There is a lack of methods for high-throughput functional microRNA (miRNA) profiling. In this chapter, we describe a recombinant adeno-associated virus-based miRNA sensor array (miRNA Asensor array), which is able to profile functional miRNAs in cultured cells. The preparation of an miRNA Asensor array and its usage are discussed.

Details

Language :
English
ISSN :
1940-6029
Volume :
1039
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
24026702
Full Text :
https://doi.org/10.1007/978-1-62703-535-4_21