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Solution properties and in vitro anti-tumor activities of polysaccharides from longan pulp.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2013 Sep 18; Vol. 18 (9), pp. 11601-13. Date of Electronic Publication: 2013 Sep 18. - Publication Year :
- 2013
-
Abstract
- The solution properties of four fractions (LPI-IV) from crude longan pulp polysaccharides (LP3) were analyzed by size-exclusion chromatography combined with laser light scattering, viscometry, complex formation with Congo red, and atomic force microscopy. Their radii of gyration <S²>(z)(½) were 43.3, 62.6, 43.2 and 77.3 nm, exponents of <S²>(z)(½) = k M(w)(v) were 0.04, 0.50, 0.52 and 0.02, and intrinsic viscosities ([η]) were 9.945, 25.38, 308.2 and 452.1 mL/g, respectively. Moreover, the dependence of [η] on M(w) was established to be [η] = 5.3 × 10⁻²M(w)⁰·⁶¹ (mL/g). LPI had both a sphere-like conformation and a triple-helix structure, and LPII-IV existed as flexible chains. LP3, LPI, LPII and LPIII all exhibited direct inhibitory effects on A549, HeLa and HepG2 cells in a positive dose-dependent manner in the range of 50-400 µg/mL. The activities of LPIII, especially the inhibition of HepG2 cell proliferation, were stronger than those of others, which may be partly related to its flexible conformation. The present results support the cancer therapeutic potential of longan polysaccharides.
- Subjects :
- Antineoplastic Agents, Phytogenic chemistry
Carbohydrate Conformation
Cell Proliferation drug effects
Drug Screening Assays, Antitumor
HeLa Cells
Hep G2 Cells
Humans
Molecular Weight
Plant Extracts chemistry
Polysaccharides chemistry
Polysaccharides ultrastructure
Solutions
Viscosity
Antineoplastic Agents, Phytogenic pharmacology
Fruit chemistry
Plant Extracts pharmacology
Polysaccharides pharmacology
Sapindaceae chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 18
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 24051475
- Full Text :
- https://doi.org/10.3390/molecules180911601