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Development of a coordinated allo T cell and auto B cell response against autosomal PTK2B after allogeneic hematopoietic stem cell transplantation.
- Source :
-
Haematologica [Haematologica] 2014 Feb; Vol. 99 (2), pp. 365-9. Date of Electronic Publication: 2013 Oct 04. - Publication Year :
- 2014
-
Abstract
- It is well known that allo-reactive T cells play a crucial role in graft-versus-leukemia and graft-versus-host disease after allogeneic hematopoietic stem cell transplantation (alloSCT). Allo-reactive CD4(+) T cells can mediate direct cytolysis, but may also stimulate production of IgG antibodies as helper cells. Immune complexes may subsequently be processed and presented by professional antigen presenting cells and stimulate induction of specific CD8(+) T cells. As such, proteins targeted in coordinated T- and B-cell responses may represent a class of immunodominant antigens in clinical responses after alloSCT. We previously identified LB-PTK2B-1T as HLA class II restricted polymorphic antigen in a patient treated with donor lymphocyte infusion for relapsed chronic myeloid leukemia after HLA-matched alloSCT. Since PTK2B has also been described as antibody target, we here investigated whether a coordinated T- and B-cell response against PTK2B was induced. Patient serum before and after alloSCT and donor lymphocyte infusion (DLI) was screened for antibodies, and we indeed observed development of a humoral immune response against PTK2B. Antibodies against PTK2B were only found after DLI and, in contrast to the CD4(+) T cells, recognized a monomorphic region of the protein. To our knowledge, this is the first description of a coordinated allo-reactive CD4(+) T-cell and auto-reactive antibody response against an autosomal antigen.
- Subjects :
- Allografts
B-Lymphocytes metabolism
B-Lymphocytes pathology
CD4-Positive T-Lymphocytes metabolism
CD4-Positive T-Lymphocytes pathology
Female
Focal Adhesion Kinase 2 genetics
Focal Adhesion Kinase 2 metabolism
Graft vs Host Disease enzymology
Graft vs Host Disease genetics
Graft vs Host Disease pathology
Histocompatibility Antigens Class II genetics
Histocompatibility Antigens Class II immunology
Histocompatibility Antigens Class II metabolism
Humans
Immunoglobulin G blood
Isoantibodies blood
Male
B-Lymphocytes immunology
CD4-Positive T-Lymphocytes immunology
Focal Adhesion Kinase 2 immunology
Graft vs Host Disease immunology
Hematopoietic Stem Cell Transplantation
Immunoglobulin G immunology
Isoantibodies immunology
Leukemia, Myelogenous, Chronic, BCR-ABL Positive enzymology
Leukemia, Myelogenous, Chronic, BCR-ABL Positive genetics
Leukemia, Myelogenous, Chronic, BCR-ABL Positive immunology
Leukemia, Myelogenous, Chronic, BCR-ABL Positive pathology
Leukemia, Myelogenous, Chronic, BCR-ABL Positive therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1592-8721
- Volume :
- 99
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Haematologica
- Publication Type :
- Academic Journal
- Accession number :
- 24097630
- Full Text :
- https://doi.org/10.3324/haematol.2013.086652