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[Interest of the study of a not identified peak on a hemoglobin chromatogram: delta variant or inter-sample contaminations?].
- Source :
-
Annales de biologie clinique [Ann Biol Clin (Paris)] 2013 Sep-Oct; Vol. 71 (5), pp. 577-80. - Publication Year :
- 2013
-
Abstract
- D-10(®) (Bio-Rad) analyzer using cationic exchange high performance chromatography (HPLC) allows the detection of the main hemoglobin variants. This observation shows the presence of a peak on chromatogram with a low intensity and no quantifiable which can lead to different diagnosis. Inter-sample contaminations can be confused with the presence of an hemoglobin variant. This case highlights the importance of the knowledge of technicals limits for validation and clinical use of results.
- Subjects :
- Blood Chemical Analysis standards
Chromatography, High Pressure Liquid methods
Chromatography, High Pressure Liquid standards
DNA Contamination
Diagnostic Errors
False Positive Reactions
Hemoglobins genetics
Humans
Blood Specimen Collection standards
Genetic Variation
Hemoglobin, Sickle analysis
Hemoglobin, Sickle genetics
Hemoglobins analysis
Subjects
Details
- Language :
- French
- ISSN :
- 1950-6112
- Volume :
- 71
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Annales de biologie clinique
- Publication Type :
- Academic Journal
- Accession number :
- 24113442
- Full Text :
- https://doi.org/10.1684/abc.2013.0867