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Muscle organization in Caenorhabditis elegans: localization of proteins implicated in thin filament attachment and I-band organization.
- Source :
-
The Journal of cell biology [J Cell Biol] 1985 Oct; Vol. 101 (4), pp. 1532-49. - Publication Year :
- 1985
-
Abstract
- The body wall muscle cells of Caenorhabditis elegans contain an obliquely striated myofibrillar lattice that is associated with the cell membrane through two structures: an M-line analogue in the A-band and a Z-disc analogue, or dense-body, in the I-band. By using a fraction enriched in these structures as an immunogen for hybridoma production, we prepared monoclonal antibodies that identify four components of the I-band as determined by immunofluorescence and Western transfer analysis. A major constituent of the dense-body is a 107,000-D polypeptide that shares determinants with vertebrate alpha-actinin. A second dense-body constituent is a more basic and antigenically distinct 107,000-D polypeptide that is localized to a narrow domain of the dense-body at or subjacent to the plasma membrane. This basic dense-body polypeptide is also found at certain cell boundaries where thin filaments in half-bands terminate at membrane-associated structures termed attachment plaques. A third, unidentified antigen is also found closely apposed to the cell membrane in regions of not only the dense-body and attachment plaque, but also the M-line analogue. Finally, a fourth high molecular weight antigen, composed of two polypeptides of approximately 400,000-D, is localized to the I-band regions surrounding the dense-body. The attachment of the dense-body to the cell surface and the differential localization of the dense-body-associated antigens suggest a model for their organization in which the unidentified antigen is a cell surface component, and the two 107,000-D polypeptides define different cytoplasmic domains of the dense-body.
- Subjects :
- Actinin immunology
Animals
Antibodies, Monoclonal immunology
Antibody Specificity
Epitopes immunology
Fluorescent Antibody Technique
Muscle Proteins immunology
Muscles analysis
Peptides immunology
Rabbits
Sarcomeres analysis
Sarcomeres ultrastructure
Caenorhabditis anatomy & histology
Muscle Proteins isolation & purification
Muscles ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 101
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 2413045
- Full Text :
- https://doi.org/10.1083/jcb.101.4.1532