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Development of exposure assessment method based on the analysis of urinary heterocyclic amines as biomarkers by on-line in-tube solid-phase microextraction coupled with liquid chromatography-tandem mass spectrometry.
- Source :
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Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2014 Mar; Vol. 406 (8), pp. 2171-8. Date of Electronic Publication: 2013 Oct 19. - Publication Year :
- 2014
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Abstract
- Heterocyclic amines (HCAs) formed in cooked meats and fish are mutagens and carcinogens in rodents and nonprimates. Exposure to HCAs may also be a risk factor for human tumors, but the association between dietary intake and human cancer risk has not been determined. To assess recent exposure to HCAs, we developed a simple and sensitive method for measuring HCAs in urine by automated on-line in-tube solid-phase microextraction (SPME) using a Supel-Q PLOT capillary column as an extraction device, in combination with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Thirteen HCAs were separated within 15 min using a ZORBAX Eclipse XDB-C8 column and detected selectively by multiple reaction monitoring using MS/MS. This method can be applied easily to the analysis of small amounts of urine samples without any other pretreatment except for alkaline hydrolysis of bound forms of HCAs. The quantification limits of HCAs in 0.2 mL of urine samples were about 1.7-4.1 pg/mL (S/Nā=ā10). Using this method, we evaluated the exposure to HCAs in persons who consumed well-done pan-fried beef and the suitability of using urinary HCAs as exposure biomarkers. We also analyzed the ability of vegetable consumption to prevent carcinogenic risks from exposure to HCAs by measuring free and bound forms of HCAs in urine.
- Subjects :
- Animals
Biomarkers urine
Cattle
Cooking
Food Contamination analysis
Humans
Meat adverse effects
Meat analysis
Solid Phase Microextraction instrumentation
Spectrometry, Mass, Electrospray Ionization methods
Amines isolation & purification
Amines urine
Chromatography, High Pressure Liquid methods
Heterocyclic Compounds analysis
Heterocyclic Compounds isolation & purification
Solid Phase Microextraction methods
Tandem Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1618-2650
- Volume :
- 406
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Analytical and bioanalytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 24141318
- Full Text :
- https://doi.org/10.1007/s00216-013-7420-1