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On the use of [18F]DOPA as an imaging biomarker for transplanted islet mass.
- Source :
-
Annals of nuclear medicine [Ann Nucl Med] 2014 Jan; Vol. 28 (1), pp. 47-52. Date of Electronic Publication: 2013 Oct 29. - Publication Year :
- 2014
-
Abstract
- Aim: Islet transplantation is being developed as a potential cure for patients with type 1 diabetes. There is a need for non-invasive imaging techniques for the quantification of transplanted islets, as current transplantation sites are associated with a substantial loss of islet viability. The dopaminergic metabolic pathway is present in the islets; therefore, we propose Fluorine-18 labeled L-3,4-dihydroxyphenylalanine ([18F]DOPA) as a biomarker for transplanted islet mass.<br />Methods: The expression of enzymes involved in the dopaminergic metabolic pathway was investigated in both native and transplanted human islets. The specific uptake of [18F]DOPA in islets and immortalized beta cells was studied in vitro by selective blocking of dopa decarboxylase (DDC). Initial in vivo PET imaging of viable subcutaneous human islets was performed using [18F]DOPA.<br />Results: DDC and vesicular monoamine transporter 2 are co-localized with insulin in the native human pancreas, and the expression is retained after transplantation. Islet uptake of the [18F]DOPA could be modulated by inhibiting DDC, indicating that the uptake followed the normal dopaminergic metabolic pathway. In vivo imaging revealed [18F]DOPA uptake at the site of the functional islet graft.<br />Conclusion: Based on the in vitro and in vivo results presented in this study, we propose to further validate [18F]DOPA-PET as a sensitive imaging modality for imaging extrahepatically transplanted islets.
- Subjects :
- Animals
Biological Transport
Carboxylic Acids metabolism
Cattle
Cell Line
Dihydroxyphenylalanine metabolism
Dopa Decarboxylase metabolism
Gene Expression Regulation, Enzymologic
Humans
Insulin-Secreting Cells diagnostic imaging
Insulin-Secreting Cells metabolism
Islets of Langerhans cytology
Islets of Langerhans diagnostic imaging
Islets of Langerhans metabolism
Mice
Organ Size
Positron-Emission Tomography
Dihydroxyphenylalanine analogs & derivatives
Islets of Langerhans anatomy & histology
Islets of Langerhans Transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1864-6433
- Volume :
- 28
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Annals of nuclear medicine
- Publication Type :
- Academic Journal
- Accession number :
- 24166476
- Full Text :
- https://doi.org/10.1007/s12149-013-0779-4