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[TcpC induces apoptosis of human vascular endothelial cells and its mechanisms].
- Source :
-
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences [Zhejiang Da Xue Xue Bao Yi Xue Ban] 2013 Sep; Vol. 42 (5), pp. 492-7. - Publication Year :
- 2013
-
Abstract
- Objective: To investigate the effects of TcpC on human umbilical vascular endothelial cells (HUVECs) and its mechanisms.<br />Methods: HUVECs were co-cultured with TcpC secreting wild-type E. coli strain CFT073 (TcpC(wt)) or tcpc gene-deleted CFT073 mutant strain (TcpC(mut)) in transwell system,respectively. Apoptosis of HUVECs was analyzed by Annexin-V/PI double staining. Mitochondrial membrane depolarization was detected by JC-1 staining. Expression of apoptosis-related proteins in HUVECs was determined by Western blot.<br />Results: HUVECs showed morphological changes after co-cultured with TcpC(wt) for 24 h: the cells became detached and cell debris increased,and cell number was also decreased when compared to HUVECs co-cultured with TcpC(mut). The apoptosis of HUVEC cells co-cultured with TcpC(wt) for 24 h significantly increased,compared to that of control group and TcpC(mut) group (60.1% 9.7% compared with 9.0% 1.3% and 16.9% 0.4%,respectively, P<0.05); meanwhile the mitochondrial depolarization of HUVECs co-cultured with TcpC(wt) was significantly increased,compared to that in control and TcpC(mut) groups (64.5% 0.9% compared with 14.5% 2.1% and 15.6% 3.3%, respectively,P<0.05). Cleavage of PARP and inhibition of Mcl-1 and XIAP expression were seen in HUVECs co-cultured with TcpC(wt),but not in groups of control and TcpC(mut).<br />Conclusion: TcpC secreted from CFT073 can induce apoptosis of HUVECs through mitochondrial pathway, in which PARP is cleaved and Mcl-1 and XIAP expressions are inhibited.
- Subjects :
- Cells, Cultured
Escherichia coli metabolism
Humans
Membrane Potential, Mitochondrial
Myeloid Cell Leukemia Sequence 1 Protein metabolism
Poly(ADP-ribose) Polymerases metabolism
X-Linked Inhibitor of Apoptosis Protein metabolism
Apoptosis drug effects
Escherichia coli Proteins pharmacology
Human Umbilical Vein Endothelial Cells drug effects
Human Umbilical Vein Endothelial Cells pathology
Virulence Factors pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1008-9292
- Volume :
- 42
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 24167128