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MicroRNA profiling in prostate cancer--the diagnostic potential of urinary miR-205 and miR-214.
- Source :
-
PloS one [PLoS One] 2013 Oct 22; Vol. 8 (10), pp. e76994. Date of Electronic Publication: 2013 Oct 22 (Print Publication: 2013). - Publication Year :
- 2013
-
Abstract
- Prostate cancer (PCa) is the most common type of cancer in men in the United States, which disproportionately affects African American descents. While metastasis is the most common cause of death among PCa patients, no specific markers have been assigned to severity and ethnic biasness of the disease. MicroRNAs represent a promising new class of biomarkers owing to their inherent stability and resilience. In the present study, we investigated potential miRNAs that can be used as biomarkers and/or therapeutic targets and can provide insight into the severity and ethnic biasness of PCa. PCR array was performed in FFPE PCa tissues (5 Caucasian American and 5 African American) and selected differentially expressed miRNAs were validated by qRT-PCR, in 40 (15 CA and 25 AA) paired PCa and adjacent normal tissues. Significantly deregulated miRNAs were also analyzed in urine samples to explore their potential as non-invasive biomarker for PCa. Out of 8 miRNAs selected for validation from PCR array data, miR-205 (p<0.0001), mir-214 (p<0.0001), miR-221(p<0.001) and miR-99b (p<0.0001) were significantly downregulated in PCa tissues. ROC curve shows that all four miRNAs successfully discriminated between PCa and adjacent normal tissues. MiR-99b showed significant down regulation (p<0.01) in AA PCa tissues as compared to CA PCa tissues and might be related to the aggressiveness associated with AA population. In urine, miR-205 (p<0.05) and miR-214 (p<0.05) were significantly downregulated in PCa patients and can discriminate PCa patients from healthy individuals with 89% sensitivity and 80% specificity. In conclusion, present study showed that miR-205 and miR-214 are downregulated in PCa and may serve as potential non-invasive molecular biomarker for PCa.
- Subjects :
- Black or African American
Aged
Gene Expression Profiling
Humans
Male
Middle Aged
Prostatic Neoplasms diagnosis
Prostatic Neoplasms pathology
Sensitivity and Specificity
United States
White People
Biomarkers, Tumor urine
Down-Regulation
Gene Expression Regulation, Neoplastic
MicroRNAs urine
Prostatic Neoplasms urine
RNA, Neoplasm urine
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24167554
- Full Text :
- https://doi.org/10.1371/journal.pone.0076994