Polycation-assisted DNA detection by reduction triggered fluorescence amplification probe.

Authors :: Saneyoshi H
Shimada N
Maruyama A
Ito Y
Abe H
Source :: Bioorganic & medicinal chemistry letters [Bioorg Med Chem Lett] 2013 Dec 15; Vol. 23 (24), pp. 6851-3. Date of Electronic Publication: 2013 Oct 11.
Publication Year :: 2013
Abstract: We have developed a fluorescence detection system for DNA, assisted by a comb-type cationic polymer (PLL-g-DX), for accelerating the reaction turnover. The combination of fluorogenic DNA probes with a comb-type cationic polymer has been demonstrated to be an effective means of signal amplification during the detection process. The method described herein represents a simple and enzyme-free detection.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)

Subjects :: DNA Probes chemistry
DNA Probes metabolism
Organophosphorus Compounds chemistry
Oxidation-Reduction
Polyelectrolytes
Polylysine chemistry
Spectrometry, Fluorescence
Temperature
DNA analysis
Fluorescent Dyes chemistry
Polyamines chemistry

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