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Overliming detoxification of pyrolytic sugar syrup for direct fermentation of levoglucosan to ethanol.
- Source :
-
Bioresource technology [Bioresour Technol] 2013 Dec; Vol. 150, pp. 220-7. Date of Electronic Publication: 2013 Oct 08. - Publication Year :
- 2013
-
Abstract
- The application of pyrolytic sugars for biofuel production through fermentation is challenged by inhibitory contaminant compounds. Inhibition is so severe that only 0.25% sugar syrup can be used. In this study, overliming was tested as a simple detoxification method, using the Escherichia coli KO11+ lgk to directly convert levoglucosan into ethanol. After treatment with at least 14.8 g/L of Ca(OH)2, fermentation with 2% (w/v) pyrolytic sugar syrup was observed with no inhibition of ethanol production. Further investigation of treatment time and temperature showed that 8-16 h of treatment at 20°C, and 1-4 h of treatment at 60°C are necessary to obtain consistent ethanol production. The samples treated with 18.5 g/L Ca(OH)2 at 60°C for 4 h showed no inhibition at 2.5%. Multiple contaminants removed by the overliming treatment were identified. This study demonstrates that overliming is a promising method for detoxification of pyrolytic sugars for fermentation.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 150
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 24177154
- Full Text :
- https://doi.org/10.1016/j.biortech.2013.09.138