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An ophthalmic solution of a peroxisome proliferator-activated receptor gamma agonist prevents corneal inflammation in a rat alkali burn model.
- Source :
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Molecular vision [Mol Vis] 2013 Nov 01; Vol. 19, pp. 2135-50. Date of Electronic Publication: 2013 Nov 01 (Print Publication: 2013). - Publication Year :
- 2013
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Abstract
- Purpose: We clarified the effects of an ophthalmic solution of a peroxisome proliferator-activated receptor gamma (PPARγ) agonist on corneal inflammation and wound healing after alkali burn injury in rats.<br />Methods: After alkali exposure, either an ophthalmic solution with 0.1% pioglitazone hydrochloride (the PPARγ group) or vehicle (the vehicle group) was topically applied to the cornea until day 14. Histological, immunohistochemical, and real-time reverse transcription polymerase chain reaction analysis were performed.<br />Results: After alkali injury, PPARγ expression increased, with the infiltration of many inflammatory cells. The infiltration of neutrophils and macrophages started from the corneal limbus within 6 h, and developed in the corneal center by day 7, with associated neovascularization. The accumulation of α-smooth muscle actin-positive myofibroblasts and the deposition of type III collagen were noted on day 14. The histological changes were suppressed significantly by treatment with the ophthalmic solution of the PPARγ agonist. In addition, the number of infiltrating M2 macrophages in the cornea was increased by PPARγ agonist treatment. In real-time reverse transcription polymerase chain reaction analysis, the messenger ribonucleic acid expression levels of interleukin-1β (IL-1β), IL-6, IL-8, monocyte chemoattractant protein-1, tumor necrosis factor-α, transforming growth factor beta 1, and vascular endothelial growth factor-A were decreased in the PPARγ group compared to the vehicle group in the early periods of corneal inflammation.<br />Conclusions: The ophthalmic solution of the PPARγ agonist inhibited inflammation, decreased the fibrotic reaction, and prevented neovascularization in the cornea from the early phase after alkali burn injury. The ophthalmic solution of the PPARγ agonist may provide a new treatment strategy with useful clinical applications for corneal inflammation and wound healing.
- Subjects :
- Alkalies
Animals
Burns, Chemical complications
Burns, Chemical pathology
Chemokines genetics
Chemokines metabolism
Collagen Type III metabolism
Cornea drug effects
Corneal Neovascularization drug therapy
Corneal Neovascularization pathology
Corneal Opacity complications
Corneal Opacity drug therapy
Corneal Opacity pathology
Disease Models, Animal
Eye Burns complications
Eye Burns pathology
Fibrosis
Inflammation complications
Inflammation drug therapy
Macrophages drug effects
Macrophages pathology
Male
Neutrophil Infiltration drug effects
PPAR gamma metabolism
Pioglitazone
RNA, Messenger genetics
RNA, Messenger metabolism
Rats
Rats, Wistar
Thiazolidinediones pharmacology
Thiazolidinediones therapeutic use
Wound Healing drug effects
Burns, Chemical drug therapy
Cornea pathology
Eye Burns drug therapy
Inflammation prevention & control
Ophthalmic Solutions pharmacology
Ophthalmic Solutions therapeutic use
PPAR gamma agonists
Subjects
Details
- Language :
- English
- ISSN :
- 1090-0535
- Volume :
- 19
- Database :
- MEDLINE
- Journal :
- Molecular vision
- Publication Type :
- Academic Journal
- Accession number :
- 24194635