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Carvedilol, a third-generation β-blocker prevents oxidative stress-induced neuronal death and activates Nrf2/ARE pathway in HT22 cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2013 Nov 29; Vol. 441 (4), pp. 917-22. Date of Electronic Publication: 2013 Nov 08. - Publication Year :
- 2013
-
Abstract
- Carvedilol, a nonselective β-adrenoreceptor blocker with pleiotropic activities has been shown to exert neuroprotective effect due to its antioxidant property. However, the neuroprotective mechanism of carvedilol is still not fully uncovered. Nuclear factor E2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway is an important cellular stress response pathway involved in neuroprotection. Here we investigated the effect of carvedilol on oxidative stress-induced cell death (glutamate 2mM and H2O2 600 μM) and the activity of Nrf2/ARE pathway in HT22 hippocampal cells. Carvedilol significantly increased cell viability and decreased ROS in HT22 cells exposed to glutamate or H2O2. Furthermore, carvedilol activated the Nrf2/ARE pathway in a concentration-dependent manner, and increased the protein levels of heme oxygenase-1(HO-1) and NAD(P)H quinone oxidoreductase-1(NQO-1), two downstream factors of the Nrf2/ARE pathway. Collectively, our results indicate that carvedilol protects neuronal cell against glutamate- and H2O2-induced neurotoxicity possibly through activating the Nrf2/ARE signaling pathway.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Antioxidant Response Elements
Carvedilol
Cell Line
Cell Survival drug effects
Glutamic Acid pharmacology
Heme Oxygenase-1 metabolism
Hydrogen Peroxide pharmacology
Mice
NF-E2-Related Factor 2 metabolism
Neurons physiology
Oxidative Stress genetics
Reactive Oxygen Species metabolism
Signal Transduction
Adrenergic beta-Antagonists pharmacology
Carbazoles pharmacology
Neurons drug effects
Neuroprotective Agents pharmacology
Oxidative Stress drug effects
Propanolamines pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 441
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 24216104
- Full Text :
- https://doi.org/10.1016/j.bbrc.2013.10.160