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Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production.
- Source :
-
Metabolic engineering [Metab Eng] 2014 Jan; Vol. 21, pp. 17-25. Date of Electronic Publication: 2013 Nov 09. - Publication Year :
- 2014
-
Abstract
- The thermophilic anaerobe Thermoanaerobacterium saccharolyticum JW/SL-YS485 was investigated as a host for n-butanol production. A systematic approach was taken to demonstrate functionality of heterologous components of the clostridial n-butanol pathway via gene expression and enzymatic activity assays in this organism. Subsequently, integration of the entire pathway in the wild-type strain resulted in n-butanol production of 0.85 g/L from 10 g/L xylose, corresponding to 21% of the theoretical maximum yield. We were unable to integrate the n-butanol pathway in strains lacking the ability to produce acetate, despite the theoretical overall redox neutrality of n-butanol formation. However, integration of the n-butanol pathway in lactate deficient strains resulted in n-butanol production of 1.05 g/L from 10 g/L xylose, corresponding to 26% of the theoretical maximum.<br /> (© 2013 The Authors. Published by International Metabolic Engineering Society on behalf of International Metabolic Engineering Society All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1096-7184
- Volume :
- 21
- Database :
- MEDLINE
- Journal :
- Metabolic engineering
- Publication Type :
- Academic Journal
- Accession number :
- 24216277
- Full Text :
- https://doi.org/10.1016/j.ymben.2013.10.012