Back to Search
Start Over
Proteomic analysis of knock-down HLA-G in invasion of human trophoblast cell line JEG-3.
- Source :
-
International journal of clinical and experimental pathology [Int J Clin Exp Pathol] 2013 Oct 15; Vol. 6 (11), pp. 2451-9. Date of Electronic Publication: 2013 Oct 15 (Print Publication: 2013). - Publication Year :
- 2013
-
Abstract
- Previous studies showed that aberrant HLA-G expression in trophoblast cells plays important roles in trophoblast invasion; however, the mechanisms remain to be explored. In this study, we found that suppressed HLA-G expression could dramatically decrease the mRNA and protein expression levels of matrix metalloproteinase 2 and matrix metalloproteinase 9, and in the proteome assay, there were 3 identified proteins namely, prefoldin 1, eukaryotic translation elongation factor 2 and malate dehydrogenase 2, which were verified by Western blot and known to be associated with invasion, cell cycle and cell metabolism, respectively. Collectively, our study indicated a potential involvement of HLA-G in autocrine networks that may regulate prefoldin, MMPs and trophoblast invasion at the maternal-fetal interface in human pregnancy.
- Subjects :
- Autocrine Communication
Blotting, Western
Cell Line
Down-Regulation
Electrophoresis, Gel, Two-Dimensional
Eukaryotic Initiation Factor-2 metabolism
Female
HLA-G Antigens genetics
Humans
Malate Dehydrogenase metabolism
Matrix Metalloproteinase 2 genetics
Matrix Metalloproteinase 2 metabolism
Matrix Metalloproteinase 9 genetics
Matrix Metalloproteinase 9 metabolism
Molecular Chaperones metabolism
Pregnancy
RNA Interference
RNA, Messenger metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Transfection
Cell Movement
HLA-G Antigens metabolism
Proteomics methods
Trophoblasts metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1936-2625
- Volume :
- 6
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- International journal of clinical and experimental pathology
- Publication Type :
- Academic Journal
- Accession number :
- 24228107