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ISG15 is counteracted by vaccinia virus E3 protein and controls the proinflammatory response against viral infection.

Authors :
Eduardo-Correia B
Martínez-Romero C
García-Sastre A
Guerra S
Source :
Journal of virology [J Virol] 2014 Feb; Vol. 88 (4), pp. 2312-8. Date of Electronic Publication: 2013 Nov 20.
Publication Year :
2014

Abstract

Conjugation of ISG15 inhibits replication of several viruses. Here, using an expression system for assaying human and mouse ISG15 conjugations (ISGylations), we have demonstrated that vaccinia virus E3 protein binds and antagonizes human and mouse ISG15 modification. To study ISGylation importance in poxvirus infection, we used a mouse model that expresses deconjugating proteases. Our results indicate that ISGylation restricts in vitro replication of the vaccinia virus VVΔE3L mutant but unconjugated ISG15 is crucial to counteract the inflammatory response produced after VVΔE3L infection.

Details

Language :
English
ISSN :
1098-5514
Volume :
88
Issue :
4
Database :
MEDLINE
Journal :
Journal of virology
Publication Type :
Academic Journal
Accession number :
24257616
Full Text :
https://doi.org/10.1128/JVI.03293-13