Back to Search
Start Over
Functional involvement of RFVT3/SLC52A3 in intestinal riboflavin absorption.
- Source :
-
American journal of physiology. Gastrointestinal and liver physiology [Am J Physiol Gastrointest Liver Physiol] 2014 Jan; Vol. 306 (2), pp. G102-10. Date of Electronic Publication: 2013 Nov 21. - Publication Year :
- 2014
-
Abstract
- Riboflavin, also known as vitamin B2, is transported across the biological membrane into various organs by transport systems. Riboflavin transporter RFVT3 is expressed in the small intestine and has been suggested to localize in the apical membranes of the intestinal epithelial cells. In this study, we investigated the functional involvement of RFVT3 in riboflavin absorption using intestinal epithelial T84 cells and mouse small intestine. T84 cells expressed RFVT3 and conserved unidirectional riboflavin transport corresponding to intestinal absorption. Apical [(3)H]riboflavin uptake was pH-dependent in T84 cells. This uptake was not affected by Na(+) depletion at apical pH 6.0, although it was significantly decreased at apical pH 7.4. The [(3)H]riboflavin uptake from the apical side of T84 cells was prominently inhibited by the RFVT3 selective inhibitor methylene blue and significantly decreased by transfection of RFVT3-small-interfering RNA. In the gastrointestinal tract, RFVT3 was expressed in the jejunum and ileum. Mouse jejunal and ileal permeabilities of [(3)H]riboflavin were measured by the in situ closed-loop method and were significantly reduced by methylene blue. These results strongly suggest that RFVT3 would functionally be involved in riboflavin absorption in the apical membranes of intestinal epithelial cells.
- Subjects :
- Animals
Biological Transport, Active
Cells, Cultured
HEK293 Cells
Humans
Ileum drug effects
Ileum metabolism
In Situ Hybridization
Intestinal Absorption drug effects
Intestinal Absorption genetics
Intestinal Mucosa metabolism
Jejunum drug effects
Jejunum metabolism
Membrane Transport Proteins drug effects
Methylene Blue pharmacology
Mice
RNA, Messenger biosynthesis
RNA, Messenger genetics
Real-Time Polymerase Chain Reaction
Transfection
Intestinal Absorption physiology
Membrane Transport Proteins genetics
Membrane Transport Proteins physiology
Riboflavin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1547
- Volume :
- 306
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Gastrointestinal and liver physiology
- Publication Type :
- Academic Journal
- Accession number :
- 24264046
- Full Text :
- https://doi.org/10.1152/ajpgi.00349.2013