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Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2014 Feb; Vol. 42 (4), pp. e27. Date of Electronic Publication: 2013 Nov 21. - Publication Year :
- 2014
-
Abstract
- Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m(6)A nucleoside at a particular position of long RNA molecule is challenging to observe. Here we report an easy and high-throughput method for detection of m(6)A nucleosides in RNA based on high-resolution melting analysis. The method relies on the previous knowledge of the modified nucleoside position at a particular place of RNA and allows rapid screening for conditions or genes necessary for formation of that modification.
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 42
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 24265225
- Full Text :
- https://doi.org/10.1093/nar/gkt1160