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Three-dimensional printed multiphase scaffolds for regeneration of periodontium complex.
- Source :
-
Tissue engineering. Part A [Tissue Eng Part A] 2014 Apr; Vol. 20 (7-8), pp. 1342-51. Date of Electronic Publication: 2014 Feb 06. - Publication Year :
- 2014
-
Abstract
- Tooth-supporting periodontium forms a complex with multiple tissues, including cementum, periodontal ligament (PDL), and alveolar bone. In this study, we developed multiphase region-specific microscaffolds with spatiotemporal delivery of bioactive cues for integrated periodontium regeneration. Polycarprolactione-hydroxylapatite (90:10 wt%) scaffolds were fabricated using three-dimensional printing seamlessly in three phases: 100-μm microchannels in Phase A designed for cementum/dentin interface, 600-μm microchannels in Phase B designed for the PDL, and 300-μm microchannels in Phase C designed for alveolar bone. Recombinant human amelogenin, connective tissue growth factor, and bone morphogenetic protein-2 were spatially delivered and time-released in Phases A, B, and C, respectively. Upon 4-week in vitro incubation separately with dental pulp stem/progenitor cells (DPSCs), PDL stem/progenitor cells (PDLSCs), or alveolar bone stem/progenitor cells (ABSCs), distinctive tissue phenotypes were formed with collagen I-rich fibers especially by PDLSCs and mineralized tissues by DPSCs, PDLSCs, and ABSCs. DPSC-seeded multiphase scaffolds upon in vivo implantation yielded aligned PDL-like collagen fibers that inserted into bone sialoprotein-positive bone-like tissue and putative cementum matrix protein 1-positive/dentin sialophosphoprotein-positive dentin/cementum tissues. These findings illustrate a strategy for the regeneration of multiphase periodontal tissues by spatiotemporal delivery of multiple proteins. A single stem/progenitor cell population appears to differentiate into putative dentin/cementum, PDL, and alveolar bone complex by scaffold's biophysical properties and spatially released bioactive cues.
- Subjects :
- Adolescent
Adult
Amelogenin pharmacology
Animals
Biomarkers metabolism
Bone Morphogenetic Protein 2 pharmacology
Calcification, Physiologic drug effects
Calcification, Physiologic genetics
Cells, Cultured
Collagen metabolism
Connective Tissue Growth Factor pharmacology
Dental Pulp cytology
Humans
Mice
Periodontium drug effects
Phenotype
RNA, Messenger genetics
RNA, Messenger metabolism
Regeneration drug effects
Stem Cells cytology
Stem Cells drug effects
Stem Cells metabolism
Young Adult
Periodontium physiology
Printing, Three-Dimensional
Regeneration physiology
Tissue Engineering methods
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1937-335X
- Volume :
- 20
- Issue :
- 7-8
- Database :
- MEDLINE
- Journal :
- Tissue engineering. Part A
- Publication Type :
- Academic Journal
- Accession number :
- 24295512
- Full Text :
- https://doi.org/10.1089/ten.TEA.2013.0386