Back to Search
Start Over
Chiral PNAs with constrained open-chain backbones.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2014; Vol. 1050, pp. 19-35. - Publication Year :
- 2014
-
Abstract
- Chiral open-chain PNAs have been shown to have improved properties in terms of control of helical handedness, DNA affinity, sequence selectivity, and cellular uptake. They can be synthesized either using preformed chiral monomers or by means of a submonomeric strategy. The former is preferred when only a stereogenic center is present at C-5, whereas for PNA-bearing substituents at C-2, the submonomeric approach is preferred, since racemization, generally occurring during the solid-phase synthesis, can be minimized by this procedure. Here we describe the protocols for the synthesis of PNA oligomers containing C-2- or C-5- (or both) modified monomers and a GC method for checking the optical purity of C-2-modified PNAs.
- Subjects :
- Biological Transport
Chromatography, Gas
DNA metabolism
Fluorenes chemistry
Models, Molecular
Nucleic Acid Conformation
Peptide Nucleic Acids chemical synthesis
Peptide Nucleic Acids metabolism
Permeability
RNA metabolism
Solid-Phase Synthesis Techniques
Stereoisomerism
Peptide Nucleic Acids chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1050
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 24297348
- Full Text :
- https://doi.org/10.1007/978-1-62703-553-8_3