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Flow cytometric sorting of human epidermal pure basal cell suspensions using a specific antikeratin monoclonal antibody.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 1986 Dec; Vol. 87 (6), pp. 792-4. - Publication Year :
- 1986
-
Abstract
- A method is described for flow cytometric sorting of epidermal basal cells, based on the cellular keratin content. KL1, a monoclonal antikeratin antibody specific for the 56.5 kD polypeptide present in suprabasal cells was used to distinguish suprabasal from basal cells. After the action of Triton X-100, epidermal cells were stained in suspensions with KL1 antibody, and KL1-positive cells were separated by flow cytometry from KL1-negative cells (basal cells). This makes it possible to sort and analyze pure fractions of human epidermal basal cells since no less than 99% of the KL1 sorted cells were bearing bullous pemphigoid antigen. Therefore, the use of specific antibodies to cytoplasmic antigens can be of help in sorting pure fractions of cells of a particular tissue for further studies.
Details
- Language :
- English
- ISSN :
- 0022-202X
- Volume :
- 87
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 2431073
- Full Text :
- https://doi.org/10.1111/1523-1747.ep12460312