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Activation of cathepsins B and L in mouse lymphosarcoma tissue under the effect of cyclophosphamide is associated with apoptosis induction and infiltration by mononuclear phagocytes.

Activation of cathepsins B and L in mouse lymphosarcoma tissue under the effect of cyclophosphamide is associated with apoptosis induction and infiltration by mononuclear phagocytes.

Authors :
Zhanaeva SY
Mel'nikova EV
Trufakin VA
Korolenko TA
Source :
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2013 Nov; Vol. 156 (1), pp. 86-90.
Publication Year :
2013

Abstract

We analyzed activities of lysosomal cystein cathepsins B and L in mouse LS lymphosarcoma and its drug-resistant RLS 40 strain and their correlations with the dynamics of the percentage of cells with fragmented DNA and CD14 (+) phagocytes over 3 days after cyclophosphamide injection. LS regression and inhibition of RLS 40 growth after cyclophosphamide injection were paralleled by an increase in cathepsins B and L activities in tumor tissues. The antitumor effect of cyclophosphamide associated with apoptosis intensity and protease activities were significantly higher in LS. Positive correlations between activities of cathepsins B and L and the LS tissue content of cells with fragmented DNA and CD14 (+) phagocytes and negative correlations thereof with tumor weight were detected. It seems that the increase in cathepsins B and L activities in LS tissues was caused by cyclophosphamide induction of apoptosis and depended on the level of tumor cell infiltration with mononuclear phagocytes.

Details

Language :
English
ISSN :
1573-8221
Volume :
156
Issue :
1
Database :
MEDLINE
Journal :
Bulletin of experimental biology and medicine
Publication Type :
Academic Journal
Accession number :
24319737
Full Text :
https://doi.org/10.1007/s10517-013-2284-0