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[Immune regulatory effect of citalopram on microglial cells].
- Source :
-
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2013 Dec; Vol. 29 (12), pp. 1251-3. - Publication Year :
- 2013
-
Abstract
- Objective: To investigate the effects of antidepressant citalopram on the gene expressions of tumor necrosis factor-alpha (TNF-α) and interleukin 1 beta (IL-1β), and to discuss the impacts of citalopram on p38 and c-jun N-terminal kinase (JNK) of mitogen-activated protein kinase (MAPK) family in microglial cells.<br />Methods: BV2 cells were induced by lipopolysaccharide (LPS) to produce TNF-α and IL-1β. After pretreatment with citalopram (20 μmol/L) for 4 h, the mRNA levels of TNF-α and IL-1β were measured by quantitative real-time PCR (qRT-PCR); after pretreatment for 24 h, the protein levels of TNF-α and IL-1β were analyzed by ELISA; the effects of citalopram on the phosphorylation of p38MAPK and JNK were observed after pretreatment for 30 min.<br />Results: Citalopram significantly inhibited the mRNA and protein expressions of TNF-α and IL-1β, and the phosphorylation of p38MAPK and JNK.<br />Conclusion: Citalopram may play the anti-inflammatory role by inhibiting MAPK pathway in microglial cells.
- Subjects :
- Animals
Cell Line
Interleukin-1beta genetics
MAP Kinase Signaling System drug effects
Microglia cytology
Microglia metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Tumor Necrosis Factor-alpha genetics
Antidepressive Agents pharmacology
Citalopram pharmacology
Immunologic Factors pharmacology
Microglia drug effects
Microglia immunology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-8738
- Volume :
- 29
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 24321066