Biotyping and genotyping (MLVA16) of Brucella abortus isolated from cattle in Brazil, 1977 to 2008.

Authors :: Minharro S
Silva Mol JP
Dorneles EM
Pauletti RB
Neubauer H
Melzer F
Poester FP
Dasso MG
Pinheiro ES
Soares Filho PM
Santos RL
Heinemann MB
Lage AP
Source :: PloS one [PLoS One] 2013 Dec 06; Vol. 8 (12), pp. e81152. Date of Electronic Publication: 2013 Dec 06 (Print Publication: 2013).
Publication Year :: 2013
Abstract: Brucellosis is a worldwide distributed zoonosis that causes important economic losses to animal production. In Brazil, information on the distribution of biovars and genotypes of Brucella spp. is scarce or unavailable. This study aimed (i) to biotype and genotype 137 Brazilian cattle isolates (from 1977 to 2008) of B. abortus and (ii) to analyze their distribution. B. abortus biovars 1, 2 and 3 (subgroup 3b) were confirmed and biovars 4 and 6 were first described in Brazil. Genotyping by the panel 1 revealed two groups, one clustering around genotype 40 and another around genotype 28. Panels 2A and 2B disclosed a high diversity among Brazilian B. abortus strains. Eighty-nine genotypes were found by MLVA16. MLVA16 panel 1 and 2 showed geographic clustering of some genotypes. Biotyping and MLVA16 genotyping of Brazilian B. abortus isolates were useful to better understand the epidemiology of bovine brucellosis in the region.

Subjects :: Animals
Bacterial Typing Techniques
Brazil epidemiology
Brucella abortus genetics
Brucella abortus isolation & purification
Brucellosis epidemiology
Brucellosis microbiology
Cattle
Cattle Diseases microbiology
DNA, Bacterial genetics
Genotype
Minisatellite Repeats
Zoonoses microbiology
Brucella abortus classification
Brucellosis veterinary
Cattle Diseases epidemiology
DNA, Bacterial classification
Phylogeny
Zoonoses epidemiology

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