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Effect of the combinatory mixture of Rubus coreanus Miquel and Astragalus membranaceus Bunge extracts on ovariectomy-induced osteoporosis in mice and anti-RANK signaling effect.
- Source :
-
Journal of ethnopharmacology [J Ethnopharmacol] 2014 Feb 03; Vol. 151 (2), pp. 951-9. Date of Electronic Publication: 2013 Dec 12. - Publication Year :
- 2014
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Abstract
- Ethnopharmacological Relevance: Postmenopausal osteoporosis is one of the most common disorders in women after menopause, which is linked to an estrogen deficiency and characterized by an excessive loss of trabecular bone. Rubus coreanus and Astragalus membranaceus have been used for their various pharmacological properties in Asia as a traditional medicine. The present study evaluated the anti-osteoporotic effects of the optimal combination of Rubus coreanus and Astragalus membranaceus in 7:3 mixture (RAM) in ovariectomized (OVX) mice by investigating bone biomechanical properties and the serum levels of TNF-α, osteocalcin, RANKL, OPG, and RANK-RANKL signal-related osteoclast differentiation markers.<br />Materials and Methods: A total of 36 mature female outbred ICR (Institute of cancer research) strain mice (7 weeks) were divided into 6 groups with 7 mice in each group as follows: (1) Sham-operated control mice (Sham) received daily oral phosphate-buffered-saline (PBS) of equal volumes through gavage. (2) OVX mice received a daily oral gavage of PBS (OVX). (3) OVX mice were treated daily with 50mg/kgb.w./day of RAM (4) with 100mg/kgb.w./day of RAM or (5) with 200mg/kgb.w./day of RAM via oral gavage. (6) OVX mice received i.p. injections of 17β-estradiol (E2) (0.1mg/kgb.w./day) three times per week for 12 weeks.<br />Results: Micro-CT images showed that oral administration of RAM to OVX mice prevented tibial bone loss, preserved trabecular bone microarchitecture, and improved bone biomechanical properties. RAM administration also showed recovery effects on the levels of TNF-α, OPG and RANKL concentration in OVX-states. Additionally, we found that the mechanism by which RAM elicited anti-osteoporotic effects was by down-regulating the expression of TRAF6 and NFATc1 in RANKL-RANK pathway, a route of osteoclast differentiation, followed by reducing the production of osteoclast differentiation factors, calcitonin receptors and cathepsin K.<br />Conclusions: Our research strongly suggests that RAM can be clinically used in the prevention and treatment of postmenopausal osteoporosis.<br /> (© 2013 Published by Elsevier Ireland Ltd.)
- Subjects :
- Animals
Cathepsin K genetics
Drug Therapy, Combination
Female
Fruit
Humans
Mice
Mice, Inbred ICR
NFATC Transcription Factors genetics
Osteocalcin blood
Osteoprotegerin blood
Ovariectomy
Phytotherapy
Plant Roots
RANK Ligand blood
Radiography
Receptors, Calcitonin genetics
TNF Receptor-Associated Factor 6 genetics
Tibia diagnostic imaging
Tibia drug effects
Tumor Necrosis Factor-alpha blood
Astragalus propinquus
Osteoporosis, Postmenopausal metabolism
Osteoporosis, Postmenopausal prevention & control
Plant Extracts pharmacology
Plant Extracts therapeutic use
Rosaceae
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7573
- Volume :
- 151
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 24333364
- Full Text :
- https://doi.org/10.1016/j.jep.2013.12.008