[Does magnetic stimulation affect wound healing? In vitro studies].

Background: Variable magnetic field of low frequency (200-300 Hz) is one of physical methods used in reducing pain as well as regeneration of bone and soft tissue. In medical literature there are case reports about successful treatment of chronic wound healing with this method. However, there is a lack of research that could explain the mechanism of action of magnetic field in this area. Literature data show that magnetic fields have an influence on cells cultures in vitro. Cells reaction depends on cells line, field parameters and time of exposition.
Objective: In our study we checked if the magnetic field of 180-195 Hz frequency influences Balb 3T3 cells viability.
Material and Methods: This study was conducted on mouse fibroblast Balb 3T3 cells, and the influence of variable magnetic field on cells was checked. Magnetic field was generated by Viofor JPS System Classic (Med&Life). Cells were seeded on 96-well plates. After 24 hours the cells culture was exposed on magnetic fields. Two controls and six groups was included in the study. Two programs generated by Viofor JPS System Classic were chosen: M1P2 and M2P2, as well as two intensities 6 and 12. Groups 1, 2, 5 and 6 were exposed once within two days, groups 3 and 4 were exposed three times a day every hour within two days. Experiment lasted two days and was repeated 3-5 times.
Results: Experiment was evaluated with colorimetric MTT test. The test showed influence of magnetic field generated by Viofor JPS System Classic on viability of Balb 3T3 cells. Three from six chosen programs resulted in the increase of viability, compare to control. The control was taken as 100%. In groups 139%, 128%, 108% and 92% of viability was noted. Results were statisticaly significant in four groups (p < 0.05, Student's t test).
Conclusions: The influence of magnetic fields generated by Viofor JPS System Classic (Med&Life) on mouse fibroblast Balb 3T3 cells was noted. Results suggest potential beneficial effect of this physical method on chronic wound treatment.